Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine

Primary TargetA specific substrate for pancreatic elastase

General description

A specific substrate for pancreatic elastase (k_m = 100 µM; k_cat/ K_m = 35,000 M^-1 s^-1 for rat pancreatic elastase, K_m = 30 µM; k_cat/K_m = 35,000 M^-1 s^-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.

A specific substrate for pancreatic elastase (K_m = 100 µM; K_cat/K_m = 35,000 s^-1 M^-1 for rat pancreatic elastase; K_m = 30 µM; K_cat/K_m = 351,000 s^-1 M^-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.r>r>Suggested protocol: see Largman, C. (1983)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Largman, L. 1983. Biochemistry22, 3763.Del Mar, E.G., et al. 1980. Biochemistry19, 468.

Packaging

5 mg in Plastic ampoule

Physical form

45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.

Sequence

Suc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)

Warning

Toxicity: Standard Handling (A)